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iGEM 2019 St Andrews - Part Development

The Thermatoga maritima Beta-glucosidase A enzyme expresses well under the T7 promoter in a pET28a, even without IPTG induction, due to a leaky promoter. The protein is mostly soluble based on small scale expression tests and runs at ~55 kDa on an SDS-Page gel.

Figure 1. Soluble expression for the wild-type protein, the pET28a vector was known to have a leaky promoter so the bands at ~55kDa (expected mass 52kDa) indicate expression, as they are absent in lanes used for mOrange and Antibody expression (lanes 2-10).

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