Part:BBa_K2929003:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

iGEM 2019 St Andrews - Part Development

The Thermatoga maritima Beta-glucosidase A enzyme expresses well under the T7 promoter in a pET28a, even without IPTG induction, due to a leaky promoter. The protein is mostly soluble based on small scale expression tests and runs at ~55 kDa on an SDS-Page gel.

Figure 1. Soluble expression for the wild-type protein, the pET28a vector was known to have a leaky promoter so the bands at ~55kDa (expected mass 52kDa) indicate expression, as they are absent in lanes used for mOrange and Antibody expression (lanes 2-10).

User Reviews

UNIQ9ef0e25d9b655201-partinfo-00000001-QINU UNIQ9ef0e25d9b655201-partinfo-00000002-QINU